Team:Chiba/Calendar-Home/20 October 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Receivers)
(→Team:Receivers)
Line 7: Line 7:
Transformation
Transformation
-
#BBa_T9002(sensitive mutant LuxR)
+
#[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002][https://2008.igem.org/Team:Chiba/Experiments:LuxR_mutant (sensitive mutant LuxR)]
-
#BBa_T9002[http://partsregistry.org/Part:BBa_T9002　BBa_T9002]
+
#[http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002]
#BBa_T9002(RBS0.6)
#BBa_T9002(RBS0.6)

Revision as of 01:44, 30 October 2008

>Home | Notebook

19 October 2008 <|> 21 October 2008

Laboratory work

Team:Receivers

Transformation

  1. [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002](sensitive mutant LuxR)
  1. [http://partsregistry.org/Part:BBa_T9002%E3%80%80BBa_T9002 BBa_T9002]
  1. BBa_T9002(RBS0.6)
  1. Plac-his aiiA-T

Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A

  1. Ptet-RBS-luxR-T-Plux-RBS-GFP-Cm-p15A
  1. Ptet-RBS-LuxR-Kan-P15A

Plux-RBS-GFP-T-Amp-colE1

  1. Ptet-RBS-LuxR-T-Plux-RBS-GFP-T-Plac-aiiA-Amp-colE1

-->Transformation


Team:Demo-Rs


  1. Receiver(T9002) pre-incubation
    1. Receiver:BBa_T9002(JW1908)wascultured in 2mL LB-Amp (37°C,12h)

~21 October

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/20_October_2008"