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Team:University of Ottawa/24 June 2008
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::<li> Genomic DNA concentration of colony A was found to be 830.125 ng/ul - that colony was then chosen for PCR amplification of the PTP2 cassette. | ::<li> Genomic DNA concentration of colony A was found to be 830.125 ng/ul - that colony was then chosen for PCR amplification of the PTP2 cassette. | ||
::<li> The PCR amplification will be performed tomorrow morning. | ::<li> The PCR amplification will be performed tomorrow morning. | ||
| + | '''Dan''' | ||
| + | :'''Miniprep | ||
| + | ::<li> Performed a miniprep to obtain pSSA42, S1, D12, and T123 plasmids. | ||
| + | ::<li> Taking the absorbances of the DNA indicated good concentrations. | ||
| + | ::<li> Digested the S1, D12 and T123 plasmids with NcoI and EcoRI | ||
| + | ::<li> Digested pSSA42 with HindIII | ||
| + | ::<li> Ran both plasmids on a gel and obtained expected results but forgot to add marker :( | ||
| + | ::<li> Redigested plasmids overnight, will run gel again tomorrow | ||
Revision as of 14:14, 30 June 2008
Contents |
Today in the Lab
Matt
- Genomic DNA extraction
- Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
- Genomic DNA concentration of colony A was found to be 830.125 ng/ul - that colony was then chosen for PCR amplification of the PTP2 cassette.
- The PCR amplification will be performed tomorrow morning.
Dan
- Miniprep
- Performed a miniprep to obtain pSSA42, S1, D12, and T123 plasmids.
- Taking the absorbances of the DNA indicated good concentrations.
- Digested the S1, D12 and T123 plasmids with NcoI and EcoRI
- Digested pSSA42 with HindIII
- Ran both plasmids on a gel and obtained expected results but forgot to add marker :(
- Redigested plasmids overnight, will run gel again tomorrow
