Team:University of Ottawa/25 June 2008

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(Difference between revisions)
(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the n...)
Line 8: Line 8:
:'''Glycerol Stock'''
:'''Glycerol Stock'''
::<li>Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.
::<li>Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.
 +
'''Dan'''
 +
:'''Ran gel of pSSA42, S1, D12, T123 plasmids'''
 +
::<li> This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
 +
:'''Cleaned out DNA box'''
 +
::<li> Cleaned out all DNA that will not be used from our box
 +
::<li> Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
 +
:::597        > 7B  > 7
 +
:::598        > 8B  > 8
 +
:::S1          > SB    > S
 +
:::D12        > DA  > D
 +
:::T123      > TB    > T
 +
:::pSSA42  > 42B  > 42
 +
:'''PCR amplification of S, D, T, 7 and 8'''
 +
::<li> S, D, and T plasmids were PCR amplified using primers F69 and F70

Revision as of 14:28, 30 June 2008

Contents

Today in the Lab

Matt

PCR Amplification
  • F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
  • I decided to use the Kaern Lab protocol for PCR Amplification.
  • The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
    • Glycerol Stock
  • Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.

    • Dan

    Ran gel of pSSA42, S1, D12, T123 plasmids
  • This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
    • Cleaned out DNA box
  • Cleaned out all DNA that will not be used from our box
  • Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
    597 > 7B > 7
    598 > 8B > 8
    S1 > SB > S
    D12 > DA > D
    T123 > TB > T
    pSSA42 > 42B > 42
    • PCR amplification of S, D, T, 7 and 8
  • S, D, and T plasmids were PCR amplified using primers F69 and F70
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/25_June_2008"