From 2008.igem.org
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| - | <div id="maincontent" style="margin-top:200px;">
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| - | =Results of Modeling=
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| - | ==Lactose Induced GFP production==
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| - | [[Image:GFP_LACTOSE.jpg|850px| GFP Production Model]]
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| - | ==E7 production system==
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| - | [[Image:Lactose.jpg|850px| E7 Production Model]]
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| - | [[Image:Varying_lactose_input.jpg|850px| E7 Production Modeling Results]]
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| - | Modeling of the System shows that Lactose induction is essential to produce E7 and a variation of Lactose input can result in different yields of E7
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| - | ==Lysis Production system==
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| - | [[Image:Lysis.jpg|850px| Lysis Production Model]]
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| - | [[Image:Varying_Lysis.jpg|850px|Lysis Prodcution Modeling Results]]
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| - | Here we define 1 as a certain threshold e.g. (<250 µM) that when the lysis protein reaches it, lysis in the cell definitely occurs.
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| - | Although we wish for an absolute AND gate, where 0 will have no lysis production at all, simulation on biological systems shows that such results are impossible. Both addition Fe ions and Ai-2 alone would induce a certain level of lysis production. However when both are available, the lysis protein production would be higher.
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| - | In the presence of Ai-2 alone, Lysis will most likely still occur albeit at a slower rate compared to situation when both Fe and Ai-2 are present.
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| - | With the addition of iron and Ai-2 together the rate of lysis production is still significantly much higher compared to Ai-2 alone.
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| - | Therefore, by giving the logical output ‘1’ as a suitable threshold value of Lysis protein production higher than that of Ai-2 induction alone, we would still be able to obtain an AND gate based on our definition.
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Latest revision as of 15:09, 30 June 2008
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