"
Minnesota/30 June 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs) |
Emartin9808 (Talk | contribs) |
||
| Line 2: | Line 2: | ||
|- | |- | ||
| - | |1. '''Set up sequencher:''' Need to determine what will be synthesized | + | |1. '''Set up sequencher:''' Need to determine what will be synthesized and research p22 mnt sequence. |
|- | |- | ||
|2. '''Repick miniprep plate #3''' | |2. '''Repick miniprep plate #3''' | ||
|- | |- | ||
|3. '''Redo glycerol stock #7''' | |3. '''Redo glycerol stock #7''' | ||
| + | |- | ||
| + | |4. '''Discuss vectors''' | ||
| + | |- | ||
| + | |5. '''Use Vector NTI''': Choose the primer base pairs (essentially 20 b.p.'s long to lessen chance of hairpins or errors). | ||
|} | |} | ||
Revision as of 15:34, 30 June 2008
| 1. Set up sequencher: Need to determine what will be synthesized and research p22 mnt sequence. |
| 2. Repick miniprep plate #3 |
| 3. Redo glycerol stock #7 |
| 4. Discuss vectors |
| 5. Use Vector NTI: Choose the primer base pairs (essentially 20 b.p.'s long to lessen chance of hairpins or errors). |
