Team:University of Ottawa/25 June 2008

From 2008.igem.org

(Difference between revisions)
Line 21: Line 21:
|-
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| 598 || 8B || 8
| 598 || 8B || 8
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| 600 || 0A || 0
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| 601 || 1C || 1
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| S1 || SB || S
| S1 || SB || S
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:'''PCR amplification of S, D, T, 7 and 8'''
:'''PCR amplification of S, D, T, 7 and 8'''
::<li> S, D, and T plasmids were PCR amplified using primers F69 and F70
::<li> S, D, and T plasmids were PCR amplified using primers F69 and F70
 +
::<li> The 600 and 601 plasmids were amplified according to the following chart: (Primer F66 is

Revision as of 17:16, 30 June 2008

Contents

Today in the Lab

Matt

PCR Amplification
  • F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
  • I decided to use the Kaern Lab protocol for PCR Amplification.
  • The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
    • Glycerol Stock
  • Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.

    • Dan

    Ran gel of pSSA42, S1, D12, T123 plasmids
  • This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
    • Cleaned out DNA box
  • Cleaned out all DNA that will not be used from our box
  • Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
    • Plasmid Strain New Name
    587 7B 7
    598 8B 8
    600 0A 0
    601 1C 1
    S1 SB S
    D12 DA D
    T123 TB T
    pSSA42 42B 42
    PCR amplification of S, D, T, 7 and 8
  • S, D, and T plasmids were PCR amplified using primers F69 and F70
  • The 600 and 601 plasmids were amplified according to the following chart: (Primer F66 is
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/25_June_2008"