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Team:Rice University/CONSTRUCTS
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==='''Constructs'''=== | ==='''Constructs'''=== | ||
| - | <p>''Saccharomyces cerevisiae'' is widely used for baking and brewing, is a versatile eukaryotic model system, and is particularly useful for synthesizing metabolites under fermentation conditions. The microaerobic conditions of fermentation impede the oxidation of sensitive bioreactive compounds and are optimal for the ''de novo'' synthesis of resveratrol. To achieve our project and expand the synthetic biology toolbox | + | <p>''Saccharomyces cerevisiae'' is widely used for baking and brewing, is a versatile eukaryotic model system, and is particularly useful for synthesizing metabolites under fermentation conditions. The microaerobic conditions of fermentation impede the oxidation of sensitive bioreactive compounds and are optimal for the ''de novo'' synthesis of resveratrol. To achieve our project goals and expand the yeast synthetic biology toolbox, we have constructed BioBricks encoding 3 yeast promoters, 3 yeast terminators, a two micron origin of replication, 2 selectable markers, 2 metabolic enzymes, and a yeast integration plasmid. We have also submitted two additional parts representing a foundational tool, including a gene encoding an amber suppressed RFP biobrick for screening of SupF+ (Amber suppressor) genotype and an amber suppressor tRNA biobrick. <br /> |
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Revision as of 03:32, 30 October 2008
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OUR TEAM ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK ::: GALLERY |
