Team:University of Ottawa/26 June 2008
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(New page: __TOC__ '''Dan''' :'''PCR gel''' ::<li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and pu...)
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(New page: __TOC__ '''Dan''' :'''PCR gel''' ::<li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and pu...)
Newer edit →
Revision as of 18:04, 30 June 2008
Dan
- PCR gel
- The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify.
- After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products.
- S&D&T amplification products showed low DNA concentration, however it is still