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Team:NYMU-Taipei/Project/pLac
From 2008.igem.org
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== Calibration of pLac (R0010) == | == Calibration of pLac (R0010) == | ||
Revision as of 05:41, 30 October 2008
Contents |
Methodology
Calibration of pLac (R0010)
pLac + E0240 under 0 and 50uM IPTG
pLac + E0240 under 50, 500 and 1,000uM IPTG
References
- [http://science.nhmccd.edu/biol/operon.html gene regulation and operon]
- [http://rothlab.ucdavis.edu/protocols/beta-galactosidase-3.html b-Galactosidase Activity Assay]
- [http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Causey/pET.html The pET Expression System]
- [http://www.escience.ws/b572/L11/L11.htm The lac operon]
- [http://www.springerlink.com/content/8texkfla66v0hcxc/ The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens, Current Microbiology 1997]
- [http://www.pnas.org/content/104/14/6043.abstract Combinatorial transcriptional control of the lactose operon of Escherichia coli, PNAS 2007 (Thomas Kuhlman and et. al)]
