Team:Chiba/Calendar-Home/25 August 2008

From 2008.igem.org

(Difference between revisions)
(Team:Input)
(Team:Communication)
Line 38: Line 38:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>5</td><td>5</td><td>1</td><td>1</td>
<td>5</td><td>5</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRI</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeI</td>
+
<td>SpeI(&mu;L)</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
</tr>
</tr>
Line 54: Line 54:
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 3</td>
+
<td>Buffer 3(&mu;L)</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
<td>-</td><td>-</td><td>1</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
<td>1</td><td>1</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2</td><td>2</td><td>7.5</td><td>-</td>
<td>2</td><td>2</td><td>7.5</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>10μl</td><td>10μl</td><td>10μl</td><td>1μl</td>
+
<td>10</td><td>10</td><td>10</td><td>1</td>
</tr>
</tr>
</table>
</table>
Line 81: Line 81:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>10</td><td>1</td>
<td>10</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td><td>1</td>
<td>2</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>-</td><td>4</td>
<td>-</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>12μl</td><td>6μl</td>
+
<td>12</td><td>6</td>
</tr>
</tr>
</table>
</table>
Line 117: Line 117:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>33ng/μl×60μl</td><td>33ng/μl×60μl</td><td>100ng/μl×20μl</td>
<td>33ng/μl×60μl</td><td>33ng/μl×60μl</td><td>100ng/μl×20μl</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstI</td>
+
<td>PstI(&mu;L)</td>
<td>2</td><td>2</td><td>2</td>
<td>2</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaI</td>
+
<td>XbaI(&mu;L)</td>
<td>2</td><td>2</td><td>-</td>
<td>2</td><td>2</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeI</td>
+
<td>SpeI(&mu;L)</td>
<td>-</td><td>-</td><td>2</td>
<td>-</td><td>-</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 2</td>
+
<td>Buffer 2(&mu;L)</td>
<td>-</td><td>-</td><td>5</td>
<td>-</td><td>-</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 3</td>
+
<td>Buffer 3(&mu;L)</td>
<td>10</td><td>10</td><td>-</td>
<td>10</td><td>10</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>10</td><td>10</td><td>5</td>
<td>10</td><td>10</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>16</td><td>16</td><td>16</td>
<td>16</td><td>16</td><td>16</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>100μl</td><td>100μl</td><td>52μl</td>
+
<td>100</td><td>100</td><td>52</td>
</tr>
</tr>
</table>
</table>

Revision as of 06:12, 30 October 2008

>Home | Notebook

24 August 2008 <|> 26 August 2008

Contents

Laboratory work

Team:Input

The following glycerol Stock was made.

[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](insert check OK)


Picked the colony and incubate with 2mL of LB-Ampicillin Medium for 12 hours at 37 degrees. Took 180μl from culture,and mixed with 880μlLB-Amp Medium.(OD=0.485)

Liquid culture(OD=0.495) 0.67μl
60%Glycerol 0.33μl
20%Glycerol Stock 1.00ml

Team:Communication

(24/8)--->Digestion
  1. Double Digestion:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  2. Double Digesiton:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
  3. Single Digestion:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1234
Sample DNA(μL) 5511
EcoRI(μL) 0.50.5--
SpeI(μL) 0.50.5--
Buffer 1 11--
Buffer 3(μL) --1-
BSA(μL) 11--
dH2O(μL) 227.5-
TOTAL(μL) 1010101
--->Gel Check
Chiba-0825-1.JPG
Sample No. 1~34
Sample DNA(μL) 101
Loading Dye(μL) 21
dH2O(μL) -4
TOTAL(μL) 126
From right;
  1. Double Digestion:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  2. Double Digesiton:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) -> ?(low)
  3. Single Digestion:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) -> ?(low)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007) -> OK(low)


--->Digestion (Double Digestion)

  1. [http://partsregistry.org/Part:BBa_C0178 BBa_C0178]
  2. [http://partsregistry.org/Part:BBa_C0170 BBa_C0170]
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Sample No. 123
Sample DNA(μL) 33ng/μl×60μl33ng/μl×60μl100ng/μl×20μl
PstI(μL) 222
XbaI(μL) 22-
SpeI(μL) --2
Buffer 2(μL) --5
Buffer 3(μL) 1010-
BSA(μL) 10105
dH2O(μL) 161616
TOTAL(μL) 10010052

Team:Output

PCR

  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](1)
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2)
  • BL21(3)
  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](4)
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](5)
No. 12345
DNA tamplate 11111
FW primer 5(Venus_YFP_fwd)5(LacZ_fwd)5(LacZ_fwd)5(rLUC_fwd)5(fLuc_fwd)
VR primer 5(VR)5(LacZ_rev)5(LacZ_rev)5(VR)5(VR)
dNTPmix 1010101010
Thermo pol buffer 1010101010
DNA pol(VENT) 11111
dH2O 6868686868
TOTAL 100μl100μl100μl100μl100μl

-->Gel Check


No. 12345
DNA tamplate 11111
loading Dye 11111
dH2O 44444
TOTAL 6μl6μl6μl6μl6μl

Mini prep

  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030]
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]


Transformation

  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030]
  • PUC19
  • pGFPUV
  • pGEX Venus YFP
  • Membren Venus YFP

PCR

  • [http://partsregistry.org/Part:BBa_J63001 BBa_J63001](1)
No. 1
DNA tamplate 1
FW primer(Venus) 5
VR primer 5
dNTPmix 10
Thermo pol buffer 10
DNA pol(VENT) 1
dH2O 68
TOTAL 100μl