Team:NYMU-Taipei/Project/Phosphate/Simulations/Phosphate

From 2008.igem.org

(Difference between revisions)
(→PST alone in high phosphate condition)
(→Simulation of Phosphate Absorption in Human)
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* [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck]
* [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck]
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== Simulation of Phosphate Absorption in Human ==
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== Simulation of Phosphate Absorption ==
* assume 1g/L (dry weight) therapeutic bacteria in the human intestine
* assume 1g/L (dry weight) therapeutic bacteria in the human intestine

Revision as of 08:57, 30 October 2008

Contents

Transportation Efficiency of PIT and PST system

Pit vs. pst.png

  • [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6998957 Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli, JB 1980 (G R Willsky and M H Malamy)]
  • high affinity-low velocity Pst (phosphate specific transport) system have a Km of 0.43 +/- 0.2 microM Pi and a Vmax of 15.9 +/- 0.3 nmol of Pi (mg [dry weight]-1min-1)
  • low affinity-high velocity Pit (Pi transport) system have a Km of 38.2 +/- 0.4 microM and a Vmax of 55 +/- 1.9 nmol of Pi (mg [dry weight]-1min-1)

Phosphate Concentrations of normal and CKD patient

  • Normal adult (0.81 to 1.45 mmol/L)
  • Chronic Kidney Disease (CDK) patient (1.29 to 2.096 mmol/L)
  • [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck]

Simulation of Phosphate Absorption

  • assume 1g/L (dry weight) therapeutic bacteria in the human intestine

PST alone in high phosphate condition

PST 2000nmol pi.jpg

  • PST system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 2 hours
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