Team:NYMU-Taipei/Project/Phosphate/Simulations/Phosphate

From 2008.igem.org

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* PST system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 2 hours
* PST system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 2 hours
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[[Image:PIT 2000nmol pi.jpg.jpg|600px]]
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* PIT system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 1 hours
* PIT system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 1 hours
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* To decrease the illness caused by fast phosphate removal, we prefer to use PST system

Latest revision as of 09:16, 30 October 2008

Transportation Efficiency of PIT and PST system

Pit vs. pst.png

  • [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6998957 Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli, JB 1980 (G R Willsky and M H Malamy)]
  • high affinity-low velocity Pst (phosphate specific transport) system have a Km of 0.43 +/- 0.2 microM Pi and a Vmax of 15.9 +/- 0.3 nmol of Pi (mg [dry weight]-1min-1)
  • low affinity-high velocity Pit (Pi transport) system have a Km of 38.2 +/- 0.4 microM and a Vmax of 55 +/- 1.9 nmol of Pi (mg [dry weight]-1min-1)

Phosphate Concentrations of normal and CKD patient

  • Normal adult (0.81 to 1.45 mmol/L)
  • Chronic Kidney Disease (CDK) patient (1.29 to 2.096 mmol/L)
  • [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck]

Simulation of Phosphate Absorption

  • assume 1g/L (dry weight) therapeutic bacteria in the human intestine


PST 2000nmol pi.jpg

  • PST system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 2 hours

PIT 2000nmol pi.jpg.jpg

  • PIT system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 1 hours
  • To decrease the illness caused by fast phosphate removal, we prefer to use PST system
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