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Team:UNIPV-Pavia/Protocols/Precipitation
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Revision as of 12:56, 1 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- DNA precipitation with sodium acetate
- Antarctic Phosphatase
- Ligation
- PCR
DNA precipitation with sodium acetate
(estimated time: 1 hour)
Materials needed:
- sodium acetate
- absolute ethanol
- ethanol 70%
- previously cut plasmid
