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Team:NTU-Singapore/Notebook/01 July 2008
From 2008.igem.org
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(New page: Chin Chong *Cells that were left to grown overnight from 30/06/08 were removed from the incubator #The cells were diluted with LB medium #OD measurement was taken after dilution #Cells wer...) |
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*The plate reader was allowed to run for 12 hrs collecting RFU readings to be used to co-relate with each other | *The plate reader was allowed to run for 12 hrs collecting RFU readings to be used to co-relate with each other | ||
*Meet up with the Sales Engineer from ITS Science and Medical, to ask more about the plate reader that we are using for characterization. Questions shall be forwarded to the Product Engineer | *Meet up with the Sales Engineer from ITS Science and Medical, to ask more about the plate reader that we are using for characterization. Questions shall be forwarded to the Product Engineer | ||
| - | * | + | *To send out Business Proposal to ITS Science and Medical |
Revision as of 14:56, 1 July 2008
Chin Chong
- Cells that were left to grown overnight from 30/06/08 were removed from the incubator
- The cells were diluted with LB medium
- OD measurement was taken after dilution
- Cells were allow to an obsorbance of OD 1.2
- After which the cells were centrifuged and resuspended with M9 medium containing glycerol
- The cells in M9 medium were allowed to incubate at 37 deg for about 10 mins
- Once the cells were prepared, the characterization experiment were carried out as per before
- This time round however, we tested with the Low, Medium and High Promoters with GFP device that were sent to us in the first edition of the iGEM Newsletter, the standard promoter was also measured
- The plate reader was allowed to run for 12 hrs collecting RFU readings to be used to co-relate with each other
- Meet up with the Sales Engineer from ITS Science and Medical, to ask more about the plate reader that we are using for characterization. Questions shall be forwarded to the Product Engineer
- To send out Business Proposal to ITS Science and Medical
