"
Team:University of Ottawa/30 June 2008
From 2008.igem.org
(Difference between revisions)
Djedrysiak (Talk | contribs)
(New page: __TOC__ ==Today in the lab== '''Dan''' :'''Gel of friday-saturday ligation was run''' ::<li> Ligation bands are very faint ::<li> Decided to perfrom PCR cleanup on reaction mixture ::<li>T...)
Newer edit →
(New page: __TOC__ ==Today in the lab== '''Dan''' :'''Gel of friday-saturday ligation was run''' ::<li> Ligation bands are very faint ::<li> Decided to perfrom PCR cleanup on reaction mixture ::<li>T...)
Newer edit →
Revision as of 17:31, 1 July 2008
Contents |
Today in the lab
Dan
- Gel of friday-saturday ligation was run
- Ligation bands are very faint
- Decided to perfrom PCR cleanup on reaction mixture
- This time I used the correct amount all reactants
- Still, gel indicated no ligation after an hour
- WIll leave in PCR machine and check tomorrow
- Digestion of 0
- Plasmid 0 containing our receptor was digested overnight with EagI in order to remove GFP which is already in the plasmid and will interfere with our system.
