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User:University of Washington/1 July 2008
From 2008.igem.org
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- The remaining cultures were placed back into the 37 degree incubator to grow overnight. | - The remaining cultures were placed back into the 37 degree incubator to grow overnight. | ||
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| + | == Conjugation == | ||
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| + | ·Transform RP4 into S17-1 cells | ||
---- | ---- | ||
[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 17:55, 2 July 2008
LuxR from AraC and TetR
- AraC sequences matched the biobrick (BBa_R0080).
BioBrick Promoter Measurements
- All overnight cultures showed successful transformation by growing on plates with kanamycin.
- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.
- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.
- The remaining cultures were placed back into the 37 degree incubator to grow overnight.
Conjugation
·Transform RP4 into S17-1 cells
Team:University_of_Washington/Notebook#Notebook
