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User:University of Washington/2 July 2008
From 2008.igem.org
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== BioBrick Promoter Measurements == | == BioBrick Promoter Measurements == | ||
| - | - The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrifuged at 14,000 RPM for 3 minutes, the supernatant was | + | - The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrifuged at 14,000 RPM for 3 minutes. Then, the supernatant was decanted out, leaving only pelleted cells. |
| - | - | + | - The pelleted cells were miniprepped using a Qiagen miniprep kit. |
- 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing. | - 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing. | ||
Revision as of 22:53, 2 July 2008
BioBrick Promoter Measurements
- The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrifuged at 14,000 RPM for 3 minutes. Then, the supernatant was decanted out, leaving only pelleted cells.
- The pelleted cells were miniprepped using a Qiagen miniprep kit.
- 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing.
- The remaining purified plasmid solutions were stored at -20 degrees Celsius.
Team:University_of_Washington/Notebook#Notebook
