Team:University of Lethbridge/Notebook/GeneralLabJuly

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Revision as of 07:32, 3 July 2008

Contents

1 July 1, 2008
- 1.1 Nathan Puhl, Andrew
2 July 2, 2008
- 2.1 Nathan, Alix, Sebastian, Munima, Roxanne, Christa

July 1, 2008

Nathan Puhl, Andrew

Made 500 mL of LB agar + amp and 500 mL of Liquid LB

July 2, 2008

Nathan, Alix, Sebastian, Munima, Roxanne, Christa

Trasformed BBa_J24679( RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18).

Protocol changes:

  -3 uL of DNA
  -spin down 200 uL of cells and resuspend in 100 uL of LB; plate on LB + amp

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