Team:KULeuven/Tools/PCR

From 2008.igem.org

(Difference between revisions)
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{|
{|
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! colspan="3" style="background:#003E81; color:white" |PCR:
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! colspan="3" style="background:#003E81; color:white" |'''PCR:'''
|-valign="top"
|-valign="top"
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|Annealing (°C)
|Annealing (°C)
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
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|{{{1}}}°
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|{{{tanneal}}}°
|-bgcolor="#66FFCC"
|-bgcolor="#66FFCC"
|Time Elongation
|Time Elongation
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
-
|{{{2}}}
+
|{{{telong}}}
|-bgcolor="#66FFCC"
|-bgcolor="#66FFCC"
|Number of Cycles
|Number of Cycles
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
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|x {{{3}}}
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|x {{{ncycles}}}
|}
|}
|
|
{| cellspacing="1"
{| cellspacing="1"
|bgcolor="#66FFCC"|Buffer (5x)
|bgcolor="#66FFCC"|Buffer (5x)
-
|bgcolor="#99CCFF" colspan="2"|µL x
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|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
|-
|-
|bgcolor="#66FFCC"|MgCl<sub>2</sub> 10 µM
|bgcolor="#66FFCC"|MgCl<sub>2</sub> 10 µM
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|bgcolor="#99CCFF" colspan="2"|[]
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|bgcolor="#99CCFF" colspan="2"|{{{mgcl}}}
|-
|-
|bgcolor="#66FFCC"|dNTP 10 µM
|bgcolor="#66FFCC"|dNTP 10 µM
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|bgcolor="#99CCFF" colspan="2"|[]
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|bgcolor="#99CCFF" colspan="2"|{{{dntp}}}
|-
|-
|bgcolor="#66FFCC"|Oligo F 10 µM
|bgcolor="#66FFCC"|Oligo F 10 µM
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|bgcolor="#99CCFF"|(Number of the Fw Primer)
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|bgcolor="#99CCFF"|{{{fwprimername}}}
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|bgcolor="#99CCFF"|µL
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|bgcolor="#99CCFF"| {{{fwprimerconc}}}
|-
|-
|bgcolor="#66FFCC"|Oligo R 10 µM
|bgcolor="#66FFCC"|Oligo R 10 µM
-
|bgcolor="#99CCFF"|(Number of the Rv Primer)
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|bgcolor="#99CCFF"|{{{rvprimername}}}
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|bgcolor="#99CCFF"|µL
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|bgcolor="#99CCFF"|{{{rvprimerconc}}}
|-
|-
|bgcolor="#66FFCC"|Water
|bgcolor="#66FFCC"|Water
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|bgcolor="#99CCFF" colspan="2"|µL
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|bgcolor="#99CCFF" colspan="2"|{{{water}}}
|-
|-
|bgcolor="#66FFCC"|Polymerase
|bgcolor="#66FFCC"|Polymerase
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|bgcolor="#99CCFF" colspan="2"|
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|bgcolor="#99CCFF" colspan="2"|{{{polymerase}}}
|-
|-
|bgcolor="#66FFCC"|DNA
|bgcolor="#66FFCC"|DNA
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|bgcolor="#99CCFF" colspan="2"|
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|bgcolor="#99CCFF" colspan="2"|{{{dna}}}
|}
|}
|
|

Revision as of 11:50, 3 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
x {{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{Image}}}|30px]]
Band (0=ladder)
{{{band}}}
Retrieved from "http://2008.igem.org/Team:KULeuven/Tools/PCR"