From 2008.igem.org
(Difference between revisions)
|
|
| Line 16: |
Line 16: |
| | |Number of Cycles | | |Number of Cycles |
| | |-bgcolor="#99CCFF" | | |-bgcolor="#99CCFF" |
| - | |x {{{ncycles}}} | + | |{{{ncycles}}} |
| | |} | | |} |
| | | | | | |
Revision as of 12:04, 3 July 2008
| PCR:
|
| PCR Settings
|
| Annealing (°C)
|
| {{{tanneal}}}°
|
| Time Elongation
|
| {{{telong}}}
|
| Number of Cycles
|
| {{{ncycles}}}
|
|
| Buffer (5x)
| {{{buffer}}}
|
| MgCl2 10 µM
| {{{mgcl}}}
|
| dNTP 10 µM
| {{{dntp}}}
|
| Oligo F 10 µM
| {{{fwprimername}}}
| {{{fwprimerconc}}}
|
| Oligo R 10 µM
| {{{rvprimername}}}
| {{{rvprimerconc}}}
|
| Water
| {{{water}}}
|
| Polymerase
| {{{polymerase}}}
|
| DNA
| {{{dna}}}
|
|
| Expected Size
|
| {{{size}}}
|
| Success
|
| {{{success}}}
|
| Image (click to enlarge)
|
| [[Image:{{{Image}}}|30px]]
|
| Band (0=ladder)
|
| {{{band}}}
|
|
"
