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Team:University of Ottawa/2 July 2008
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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Ligation''' ::<li> I am using 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42. ::<li> A gel confirmation...) |
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'''Matt''' | '''Matt''' | ||
:'''Ligation''' | :'''Ligation''' | ||
| - | ::<li> I am using 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42. | + | ::<li> I am using three different samples of 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42. |
::<li> A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2. | ::<li> A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2. | ||
::<li> We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning. | ::<li> We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning. | ||
Revision as of 15:10, 3 July 2008
Contents |
Today in the Lab
Matt
- Ligation
- I am using three different samples of 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42.
- A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2.
- We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
- Transformation
- The competent cells were transformed with the ligation product and left overnight.The receptor component Atcre was also integrated into competent cells.
- Dehydrogenase component
- The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.
