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Minnesota/3 July 2008
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| + | |1. '''Make LB plates:''' Using 125mL of LB, add 125uL of kanamycin antibiotic. Pour into empty sterile agar plates, allow time to solidify. | ||
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| + | |2. '''Glycerol Stocks:''' Using 900uL of glycerol, add 900uL of transformed DH5alpha cells. Place in dry ice bath (EtOH + dry ice), allow to 'snap freeze', then place in freezer @ -79C. | ||
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Latest revision as of 16:54, 3 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 2) | Go to Next Day (July 4) |
| 1. Make LB plates: Using 125mL of LB, add 125uL of kanamycin antibiotic. Pour into empty sterile agar plates, allow time to solidify. |
| 2. Glycerol Stocks: Using 900uL of glycerol, add 900uL of transformed DH5alpha cells. Place in dry ice bath (EtOH + dry ice), allow to 'snap freeze', then place in freezer @ -79C. |
