Team:University of Ottawa/3 July 2008

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(Difference between revisions)
(Today in the Lab)
(Today in the Lab)
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:'''Competent Cells'''
:'''Competent Cells'''
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
 +
::<li> The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
:'''Ligation'''
:'''Ligation'''
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.

Revision as of 17:32, 3 July 2008

Contents

Today in the Lab

Matt

Competent Cells
  • Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
  • The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
  • The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
  • Ligation
  • Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.