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Team:University of Ottawa/3 July 2008
From 2008.igem.org
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:'''Competent Cells''' | :'''Competent Cells''' | ||
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth | ::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth | ||
| + | ::<li> The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow. | ||
::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli. | ::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli. | ||
:'''Ligation''' | :'''Ligation''' | ||
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today. | ::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today. | ||
Revision as of 17:32, 3 July 2008
Contents |
Today in the Lab
Matt
- Competent Cells
- Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
- The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
- The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
- Ligation
- Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
