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Team:MIT/Notebook/Data/toothbinding/6-30
From 2008.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
{| border="1" class="wikitable" style="text-align:center" | {| border="1" class="wikitable" style="text-align:center" | ||
| - | |+Toothbinding assay key (D=Diluted Saliva UD=Undiluted Saliva) | + | |+Toothbinding assay key with HA (D=Diluted Saliva UD=Undiluted Saliva) |
|- | |- | ||
! cells !! 5 min. !! 1h !! 2h !! 5 min. !! 1h !! 2h | ! cells !! 5 min. !! 1h !! 2h !! 5 min. !! 1h !! 2h | ||
|- | |- | ||
! 500 | ! 500 | ||
| - | | | + | | UD || UD || UD | UD || D || D || D || D | |
|- | |- | ||
! 167 | ! 167 | ||
| Line 40: | Line 40: | ||
Binding Assay (with HA beads):day 1 | Binding Assay (with HA beads):day 1 | ||
[[Image:HAbindingassay.jpg| 200px| Binding assay with HA]] | [[Image:HAbindingassay.jpg| 200px| Binding assay with HA]] | ||
| - | |||
| - | |||
| - | |||
Binding Assay (with HA beads):day 2 | Binding Assay (with HA beads):day 2 | ||
[[Image:HAassay2.jpg| 200px| Binding assay with HA]] | [[Image:HAassay2.jpg| 200px| Binding assay with HA]] | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align:center" | ||
| + | |+Toothbinding assay key without HA (D=Diluted Saliva UD=Undiluted Saliva) | ||
| + | |- | ||
| + | ! cells !! 5 min. !! 1h !! 2h !! 5 min. !! 1h !! 2h | ||
| + | |- | ||
| + | ! 500 | ||
| + | | D || D || D | D || UD || UD || UD || UD | | ||
| + | |- | ||
| + | ! 167 | ||
| + | | - || - || - | - || - || - || - | | ||
| + | |- | ||
| + | ! 56 | ||
| + | | - || - || - | - || - || - || - | | ||
| + | |- | ||
| + | ! 18 | ||
| + | | - || - || - | - || - || - || - | | ||
| + | |- | ||
| + | ! 6 | ||
| + | | - || - || - | - || - || - || - | | ||
| + | |- | ||
| + | ! 2 | ||
| + | | - || - || - | - || - || - || - | | ||
| + | |} | ||
| + | |||
| + | Binding Assay (without HA): day 1 | ||
| + | [[Image:NonHAbinding.jpg| 200px| Binding assay without HA]] | ||
| + | |||
Binding Assay (without HA): day 2 | Binding Assay (without HA): day 2 | ||
[[Image:Sailvabinding2.jpg| 200px| Binding assay without HA]] | [[Image:Sailvabinding2.jpg| 200px| Binding assay without HA]] | ||
Latest revision as of 19:17, 3 July 2008
Plated S. Mutans
.jpg)
Centrifuged plated saliva: day 2
Centrifuged plated saliva: day 3
Uncentrifuged saliva: day 2
Uncentrifuged saliva: day 3
| cells | 5 min. | 1h | 2h | 5 min. | 1h | 2h | |
|---|---|---|---|---|---|---|---|
| 500 | UD | UD | UD | D | D | D | |
| 167 | - | - | - | - | - | ||
| 56 | - | - | - | - | - | ||
| 18 | - | - | - | - | - | ||
| 6 | - | - | - | - | - | ||
| 2 | - | - | - | - | - |
Binding Assay (with HA beads):day 1
Binding Assay (with HA beads):day 2
| cells | 5 min. | 1h | 2h | 5 min. | 1h | 2h | |
|---|---|---|---|---|---|---|---|
| 500 | D | D | D | UD | UD | UD | |
| 167 | - | - | - | - | - | ||
| 56 | - | - | - | - | - | ||
| 18 | - | - | - | - | - | ||
| 6 | - | - | - | - | - | ||
| 2 | - | - | - | - | - |
Binding Assay (without HA): day 1
Binding Assay (without HA): day 2
