Team:University of Ottawa/3 July 2008

From 2008.igem.org

(Difference between revisions)
(→Today in the Lab)
Line 8: Line 8:
:'''Ligation'''
:'''Ligation'''
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
 +
'''Dan'''
 +
:'''Atcre integration'''
 +
::<li> Plate had ~4 colonies so I decided to redo integration.
 +
:'''1,2,3,4 and S,D,T ligation'''
 +
::<li> The concentration of these constructs was determined to be decent
 +
::<li> A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
 +
::<li> 1,2,3,4 did not show up on the gel for unknown reasons.
 +
 +
'''Dan and Matt'''
 +
:'''Integration'''
 +
::<li> Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated

Revision as of 21:15, 3 July 2008

Contents

Today in the Lab

Matt

Competent Cells
  • Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
  • The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
  • The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
    • Ligation
  • Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.

    • Dan

    Atcre integration
  • Plate had ~4 colonies so I decided to redo integration.
    • 1,2,3,4 and S,D,T ligation
  • The concentration of these constructs was determined to be decent
  • A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
  • 1,2,3,4 did not show up on the gel for unknown reasons.

    • Dan and Matt

    Integration
  • Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/3_July_2008"