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NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Back to Parameter Estimation

Contents 1 Parameter aD analysis 1.1 Changing aD, nD=1 1.2 Changing aD, nD=10 1.3 Changing aD, nD=100

Parameter aD analysis

In the following graphs, the red line represents the production of Lysis when both Autoinducer 2 and Iron ions are present. The blue line represents the graphs when only Autoinducer 2 is present.

Changing aD, nD=1

Graph of aD=0.00001

Graph of aD=0.001

Graph of aD=0.01

We observe that when nD = 1, changing aD has no significant effect to Lysis protein production. Also there seems to be no difference between the two forms of Lysis protein production even under different situations.

Changing aD, nD=10

Graph of aD=0.0001

Graph of aD=0.001

The graph presents a range of values aD can take on when nD = 10. As we can see, changing the values of aD has no effect on Lysis protein production as well. The difference between the Lysis protein production under the two different circumstances is now more obvious.

Changing aD, nD=100

Graph of aD=0.0001

Graph of aD=0.001

Graph of aD=0.01

Here the difference in Lysis protein production is more pronounced and obvious. We understand from these different trends that aD has little or no significant impact on protein production and that nD will be the main deciding factor.