Minnesota/4 July 2008

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Revision as of 15:38, 7 July 2008

1. PCR (Polymerase Chain Reaction): PCR used to modify Lambda_cI by copying a section of the gene, adding RBS, and using RS (restriction site enzyme) to cut copied gene section at the appropriate restriction site.
Components Volume
Phusion HF Buffer 10.0uL
10 mM dNTP's 1.0uL
Primer mix 2.5uL
Template DNA 1.0uL
Phusion DNA Polymerase 0.5uL
H20 35.0uL