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Minnesota/4 July 2008
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Emartin9808 (Talk | contribs) (New page: {| |- |1. '''PCR (Polymerase Chain Reaction):''' PCR used to modify Lambda_cI by copying a section of the gene, adding RBS, and using RS (restriction site enzyme) to cut copied gene secti...) |
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| + | ::'''PCR Components in 50uL sln''' | ||
| + | {|border="1" align="center" | ||
| + | !Sequence Name !!Forward Primer !!Reverse Primer | ||
|- | |- | ||
| - | !| Components | + | !| Components !!Volume |
|- | |- | ||
|Phusion HF Buffer ||10.0uL | |Phusion HF Buffer ||10.0uL | ||
Revision as of 15:42, 7 July 2008
| 1. PCR (Polymerase Chain Reaction): PCR used to modify Lambda_cI by copying a section of the gene, adding RBS, and using RS (restriction site enzyme) to cut copied gene section at the appropriate restriction site. |
| Sequence Name | Forward Primer | Reverse Primer |
|---|---|---|
| Components | Volume | |
| Phusion HF Buffer | 10.0uL | |
| 10 mM dNTP's | 1.0uL | |
| Primer mix | 2.5uL | |
| Template DNA | 1.0uL | |
| Phusion DNA Polymerase | 0.5uL | |
| H20 | 35.0uL |
