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Minnesota/8 July 2008
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|1. '''Simulations:''' Finish typing into SynbioSS the simulations/rxn network. | |1. '''Simulations:''' Finish typing into SynbioSS the simulations/rxn network. | ||
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| - | |2. '''Restart/Redo PCR''' | + | |2. '''Restart/Redo PCR''': Problem with original PCR was an error in the calculation of DNA concentration of product. 07-07-2008 we added only 1.0uL of PCR to digest reactions meaning only 3ng of DNA was in the solution. That was not a substantial amount of DNA to be seen in a electrophoretic gel. FIX - recalculated DNA concentration and is 3ng/1uL not 300ng/uL |
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Revision as of 15:54, 8 July 2008
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| 1. Simulations: Finish typing into SynbioSS the simulations/rxn network. |
| 2. Restart/Redo PCR: Problem with original PCR was an error in the calculation of DNA concentration of product. 07-07-2008 we added only 1.0uL of PCR to digest reactions meaning only 3ng of DNA was in the solution. That was not a substantial amount of DNA to be seen in a electrophoretic gel. FIX - recalculated DNA concentration and is 3ng/1uL not 300ng/uL |
