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User:University of Washington/8 July 2008
From 2008.igem.org
(Difference between revisions)
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| - | == BioBrick Promoter | + | == BioBrick Promoter Construct Sequencing == |
| + | |||
| + | - Because the previous forward sequencing reaction and analysis yielded high-noise results, the forward sequencing protocol was carried out a second time. | ||
- VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water. | - VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water. | ||
| - | - 8 uL of diluted VF2 primer (1pmol/uL) was added to Eppendorf tubes. | + | - 8 uL of diluted VF2 primer (1pmol/uL) was added to four Eppendorf tubes. |
| + | |||
| + | - 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriate labeling of the tubes. | ||
| - | - | + | - Plasmid template and VF2 primer solutions were resubmitted to the UW DNA Sequencing Facility for reaction and analysis. |
| - | - | + | - Fingers crossed. |
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 17:58, 8 July 2008
BioBrick Promoter Construct Sequencing
- Because the previous forward sequencing reaction and analysis yielded high-noise results, the forward sequencing protocol was carried out a second time.
- VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water.
- 8 uL of diluted VF2 primer (1pmol/uL) was added to four Eppendorf tubes.
- 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriate labeling of the tubes.
- Plasmid template and VF2 primer solutions were resubmitted to the UW DNA Sequencing Facility for reaction and analysis.
- Fingers crossed.
Back to Team:University_of_Washington/Notebook#Notebook
