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Team:University of Ottawa/8 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: __TOC__ ==Today in the Lab=== '''Matt''' :'''Digestion''' ::<li> Digestion was performed again with PstI for PTP2 ligation product.\, this time longer digestion period with more enzyme. ::...) |
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'''Matt''' | '''Matt''' | ||
:'''Digestion''' | :'''Digestion''' | ||
| - | ::<li> Digestion was performed again with PstI for PTP2 ligation product. | + | ::<li> Digestion was performed again with PstI for PTP2 ligation product. This time longer digestion period with more enzyme was used. |
::<li> Gel results still unsuccessful, I am going to try using EcoRI on Thursday instead of PstI. | ::<li> Gel results still unsuccessful, I am going to try using EcoRI on Thursday instead of PstI. | ||
| - | ::<li> Digestion was performed with Atcre using EagI | + | ::<li> Digestion was performed with Atcre using EagI, a gel extraction will then be performed tomorrow with the digestion product. |
:'''Master Plates''' | :'''Master Plates''' | ||
::<li> Master plates were made for the By4724 int DQ232597/8 so that a Quick genomic DNA isolation can be performed tomorrow. | ::<li> Master plates were made for the By4724 int DQ232597/8 so that a Quick genomic DNA isolation can be performed tomorrow. | ||
Revision as of 20:10, 8 July 2008
Contents |
Today in the Lab=
Matt
- Digestion
- Digestion was performed again with PstI for PTP2 ligation product. This time longer digestion period with more enzyme was used.
- Gel results still unsuccessful, I am going to try using EcoRI on Thursday instead of PstI.
- Digestion was performed with Atcre using EagI, a gel extraction will then be performed tomorrow with the digestion product.
- Master Plates
- Master plates were made for the By4724 int DQ232597/8 so that a Quick genomic DNA isolation can be performed tomorrow.
