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Team:NTU-Singapore/Notebook/9 July 2008
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(New page: E7 Story continued... *Ethidium Bromide Gel showed clean bands at 2k mark, took PCR direct to PCR purified to 30ul. **Gel run-smeary band..S*** What went wrong? *Trouble SHOOT SHOOT **Pro...)
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(New page: E7 Story continued... *Ethidium Bromide Gel showed clean bands at 2k mark, took PCR direct to PCR purified to 30ul. **Gel run-smeary band..S*** What went wrong? *Trouble SHOOT SHOOT **Pro...)
Newer edit →
Revision as of 10:02, 9 July 2008
E7 Story continued...
- Ethidium Bromide Gel showed clean bands at 2k mark, took PCR direct to PCR purified to 30ul.
- Gel run-smeary band..S*** What went wrong?
- Trouble SHOOT SHOOT
- Protocol different from 8 July
- Optimize Protocol
- Protocol different from 8 July
HOW? Take Gel extracted E7(50ul) direct, Gel extract(50ul)-PCR Purified to run gel to see the difference..To see the effectiveness of PCR purification and Gel Extract..Compare!
T7ptag Story 2
PCR product obtained..Ran Gel with 4ul..Good clear band Ran gel to purify the rest..loaded with 10ul
