"
Minnesota/9 July 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs) (New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 8)'''|| width=158|'''[[Mi...) |
Emartin9808 (Talk | contribs) |
||
| Line 9: | Line 9: | ||
|- | |- | ||
|1. '''Single Digests''': Performed today, refer to table below. | |1. '''Single Digests''': Performed today, refer to table below. | ||
| + | |} | ||
| + | |||
| + | {| | ||
| + | |- | ||
| + | {|border="1" align="left" | ||
| + | |- | ||
| + | !| Gene !!10x Buffer !!BSA Protein !!H20 !!DNA !!RE | ||
| + | |- | ||
| + | |Promoter ||5.0uL ||0.5uL ||40.4uL ||2.1uL ||2.0uL, SPE | ||
| + | |- | ||
| + | |LAMBDAcI ||5.0uL ||0.5uL ||29.5uL ||15.0uL ||1.0uL, XBA 1 | ||
| + | |- | ||
| + | |LAMBDAcI ||5.0uL ||0.5uL ||29.5uL ||15.0uL ||1.0uL, SPE | ||
| + | |- | ||
| + | |Terminator ||5.0uL ||0.5uL ||38.0uL ||4.5uL ||2.0uL, XBA 1 | ||
| + | |- | ||
| + | |} | ||
| + | {| | ||
| + | |- | ||
| + | |NOTE: RE = Restriction Enzyme | ||
Revision as of 15:53, 9 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 8) | Go to Next Day (July 10) |
| 1. Single Digests: Performed today, refer to table below. |
| Gene | 10x Buffer | BSA Protein | H20 | DNA | RE |
|---|---|---|---|---|---|
| Promoter | 5.0uL | 0.5uL | 40.4uL | 2.1uL | 2.0uL, SPE |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, XBA 1 |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, SPE |
| Terminator | 5.0uL | 0.5uL | 38.0uL | 4.5uL | 2.0uL, XBA 1 |
| NOTE: RE = Restriction Enzyme |
