Minnesota/9 July 2008

From 2008.igem.org

(Difference between revisions)
Line 8: Line 8:
{|
{|
|-
|-
-
|1. '''Single Digests''': Performed today, refer to table below.
+
|1. '''Single Digests''': Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below.
|}
|}
Line 26: Line 26:
|-
|-
|}
|}
 +
{|
{|
|-
|-
|NOTE: RE = Restriction Enzyme
|NOTE: RE = Restriction Enzyme
 +
|}
 +
 +
{|
 +
|-
 +
|2. '''Ligation''': Refer to table below.
 +
|}
 +
 +
{|
 +
|-
 +
{|border="1" align="left"
 +
|-
 +
!| Genes !!10x Buffer !!H20 !!Base Vector !!Insert  !!DNA Ligase
 +
|-
 +
|LAMBDAcI/Terminator || 2.0uL ||10.7uL ||2.0uL (Term.) ||4.3uL (LAMBDAcI) ||1.0uL
 +
|-
 +
|Promoter/LAMBDAcI ||2.0uL ||10.7uL ||2.0uL (Pro.) ||4.3uL (LAMBDAcI) ||1.0uL
 +
|-
 +
|}

Revision as of 16:04, 9 July 2008

Back to Notebook Home
Go to Previous Day (July 8)Go to Next Day (July 10)
1. Single Digests: Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below.
Gene 10x Buffer BSA Protein H20 DNA RE
Promoter 5.0uL 0.5uL 40.4uL 2.1uL 2.0uL, SPE
LAMBDAcI 5.0uL 0.5uL 29.5uL 15.0uL 1.0uL, XBA 1
LAMBDAcI 5.0uL 0.5uL 29.5uL 15.0uL 1.0uL, SPE
Terminator 5.0uL 0.5uL 38.0uL 4.5uL 2.0uL, XBA 1
NOTE: RE = Restriction Enzyme
2. Ligation: Refer to table below.
Genes 10x Buffer H20 Base Vector Insert DNA Ligase
LAMBDAcI/Terminator 2.0uL 10.7uL 2.0uL (Term.) 4.3uL (LAMBDAcI) 1.0uL
Promoter/LAMBDAcI 2.0uL 10.7uL 2.0uL (Pro.) 4.3uL (LAMBDAcI) 1.0uL
Retrieved from "http://2008.igem.org/Minnesota/9_July_2008"