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Minnesota/9 July 2008
From 2008.igem.org
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|1. '''Single Digests''': Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. | |1. '''Single Digests''': Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. | ||
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| - | + | NOTE: RE = Restriction Enzyme | |
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| - | + | 2. '''Ligation''': Performed today. Incubate ligated samples @ 16C for 1hour. Heat inactivated enzyme @ 65C for 15 min. Refer to table below. | |
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Revision as of 16:18, 9 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 8) | Go to Next Day (July 10) |
| 1. Single Digests: Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. |
| Gene | 10x Buffer | BSA Protein | H20 | DNA | RE |
|---|---|---|---|---|---|
| Promoter | 5.0uL | 0.5uL | 40.4uL | 2.1uL | 2.0uL, SPE |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, XBA 1 |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, SPE |
| Terminator | 5.0uL | 0.5uL | 38.0uL | 4.5uL | 2.0uL, XBA 1 |
NOTE: RE = Restriction Enzyme
2. Ligation: Performed today. Incubate ligated samples @ 16C for 1hour. Heat inactivated enzyme @ 65C for 15 min. Refer to table below.
| Genes | 10x Buffer | H20 | Base Vector | Insert | DNA Ligase |
|---|---|---|---|---|---|
| LAMBDAcI/Terminator | 2.0uL | 10.7uL | 2.0uL (Term.) | 4.3uL (LAMBDAcI) | 1.0uL |
| Promoter/LAMBDAcI | 2.0uL | 10.7uL | 2.0uL (Pro.) | 4.3uL (LAMBDAcI) | 1.0uL |
| 3. Make Ampicillin Plates |
