User:University of Washington/10 July 2008

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== RP4 Conjugation ==
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'''Conjugation protocol #1'''<br\>
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·DH5α <br\>
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·DH5α + S17-1 w/ RP4 <br\>
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·DH5α + naked RP4 DNA <br\>
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·DH5α + amp resistant RFP plasmid
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·Synthesis tetR insert for KorA and trbA replacement
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== non-RP4 Conjugation ==
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'''Mini Prep'''<br\>
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·CV13(Yep13)<br\>
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·pDPT51
----
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Revision as of 00:10, 10 July 2008

RP4 Conjugation

Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid

·Synthesis tetR insert for KorA and trbA replacement

non-RP4 Conjugation

Mini Prep
·CV13(Yep13)
·pDPT51

Back to Team:University_of_Washington/Notebook#Notebook

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