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User:University of Washington/10 July 2008
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== RP4 Conjugation == | == RP4 Conjugation == | ||
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·DH5α + naked RP4 DNA <br\> | ·DH5α + naked RP4 DNA <br\> | ||
·DH5α + amp resistant RFP plasmid | ·DH5α + amp resistant RFP plasmid | ||
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| + | == lambda red == | ||
·Synthesis tetR insert for KorA and trbA replacement | ·Synthesis tetR insert for KorA and trbA replacement | ||
Revision as of 00:11, 10 July 2008
RP4 Conjugation
Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid
lambda red
·Synthesis tetR insert for KorA and trbA replacement
non-RP4 Conjugation
Mini Prep
·CV13(Yep13)
·pDPT51
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