User:University of Washington/10 July 2008
From 2008.igem.org
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== RP4 Conjugation == | == RP4 Conjugation == | ||
- | '''Conjugation protocol #1'''<br\> | + | '''[[Protocols| Conjugation protocol #1]]'''<br\> |
·DH5α <br\> | ·DH5α <br\> | ||
·DH5α + S17-1 w/ RP4 <br\> | ·DH5α + S17-1 w/ RP4 <br\> |
Revision as of 00:22, 10 July 2008
RP4 Conjugation
Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid
Lambda Red
·Synthesis tetR insert for KorA and trbA replacement
non-RP4 Conjugation
Mini Prep & Glycerol Stock
·CV13(Yep13)
·pDPT51
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