Team:KULeuven/Tools/PCR

From 2008.igem.org

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! colspan="3" style="background:#003E81; color:white" |'''PCR:'''
! colspan="3" style="background:#003E81; color:white" |'''PCR:'''
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|style="background:#003E81; color:white"|PCR Settings
|style="background:#003E81; color:white"|PCR Settings
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|{{{ncycles}}}
|{{{ncycles}}}
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| width="200" bgcolor="#66FFCC"|Buffer (5x)
| width="200" bgcolor="#66FFCC"|Buffer (5x)
|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
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|bgcolor="#99CCFF" colspan="2"|{{{dna}}}
|bgcolor="#99CCFF" colspan="2"|{{{dna}}}
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| width="200" bgcolor="#66FFCC"|Expected Size
| width="200" bgcolor="#66FFCC"|Expected Size
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Revision as of 17:31, 11 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
{{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{image}}}|30px]]
Lane (0=ladder)
{{{lane}}}
Retrieved from "http://2008.igem.org/Team:KULeuven/Tools/PCR"