Team:Johns Hopkins/Protocols

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Protocols

Plasmid Miniprep
Ligation and Transformation
PCR Protocols from Jaime

Papers

1 Herskowitz I. A regulatory hierarchy for cell specialization in yeast. (REVIEW.) Nature. 342:749-757 (1989)

1 Johnson AD. Molecular mechanisms of cell-type determination in budding yeast. (REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)

1 Hu R-G et al. The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature. 437:981-986 (2005)

1 Varshavsky A. The N-end Rule pathway of protein degradation. (REVIEW.) Genes to Cells. 2:13-28 (1997)

1 Sheff MA & Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 21:661-670 (2004)

1 Bardwell L. A walk-through of the yeast mating pheromone response pathway. (REVIEW.) Peptides. 25:1465-1476 (2004)

1 Hill JE et al. Yeast/E. coli Shuttle Vectors with Multiple Unique Restriction Sites. Yeast. 2:163-167 (1986)

@@ Line 127: / Line 127: @@
 [[Media:JHU_0708_paper_MolecularMechanismofcell-typedeterminationinbuddingyeast.pdf|1]] Johnson AD. <i> Molecular mechanisms of cell-type determination in budding yeast. </i>(REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)<br>
-[[Media:JHU_0708_paper_The_N-end_Rule_Pathway_as_a_Nitric_Oxide_Sensor_Controlling_the_Levels_of_Multiple_Regulators.pdf|1]]  Hu R-G <I>et al.</I> <I>The N-end rule pathway as a nitric oxide sensor controlling the levels of
+[[Media:JHU_0708_paper_The_N-end_Rule_Pathway_as_a_Nitric_Oxide_Sensor_Controlling_the_Levels_of_Multiple_Regulators.pdf|1]]  Hu R-G <I>et al.</I> <I>The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators.</I> Nature. 437:981-986 (2005)<br>
-multiple regulators.</I> Nature. 437:981-986 (2005)<br>
+[[Media:JHU_0708_paper_TheN-endRule.pdf|1]] Varshavsky A. <I>The N-end Rule pathway of protein degradation. (REVIEW.)</I> Genes to Cells. 2:13-28 (1997)<br>
+[[Media:JHU_0708_paper_Optimizedcassettes.pdf|1]]  Sheff MA & Thorn KS. <I>Optimized cassettes for fluorescent protein tagging in</I> Saccharomyces cerevisiae. Yeast. 21:661-670 (2004)<br>
+[[Media:JHU_0708_paper_Awalk.pdf|1]]   Bardwell L. <I>A walk-through of the yeast mating pheromone response pathway. (REVIEW.)</I> Peptides. 25:1465-1476 (2004)<br>
@@ Line 137: / Line 141: @@
 [[Media:JHU_0708_paper_TransfofYeast.pdf|JHU_0708_paper_TransfofYeast.pdf]]<br>
 [[Media:JHU_0708_paper_Three-prongedgenomicanalysis.pdf|JHU_0708_paper_Three-prongedgenomicanalysis.pdf]]<br>
-[[Media:JHU_0708_paper_TheN-endRule.pdf|JHU_0708_paper_TheN-endRule.pdf]]<br>
 [[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf|JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf]]<br>
 [[Media:JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf|JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf]]<br>
-[[Media:JHU_0708_paper_Optimizedcassettes.pdf|JHU_0708_paper_Optimizedcassettes.pdf]]<br>
 [[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf|JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf]]<br>
 [[Media:JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf|JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf]]<br>
@@ Line 148: / Line 150: @@
 [[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf|JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf]]<br>
 [[Media:JHU_0708_paper_BentDNA.pdf|JHU_0708_paper_BentDNA.pdf]]<br>
-[[Media:JHU_0708_paper_Awalk.pdf|JHU_0708_paper_Awalk.pdf]]<br>
 [[Media:JHU_0708_paper_Aregularoryheirarchy.pdf|JHU_0708_paper_Aregularoryheirarchy.pdf]]<br>
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