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Team:ESBS-Strasbourg/Competent Bacteria
From 2008.igem.org
(Difference between revisions)
(New page: =Solutions= '''KCM 5x''' <br> 100ml<br> {|cellpadding='5' |0.5M KCl |3.7 g |KCl 74,56 g/mol |- |0.15M CaCl2 |2.2 g |CaCl2 2H2O 147,02 g/mol |- |0.25M MgCl2 |5 g ...) |
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Revision as of 12:10, 14 July 2008
Solutions
KCM 5x
100ml
| 0.5M KCl | 3.7 g | KCl 74,56 g/mol |
| 0.15M CaCl2 | 2.2 g | CaCl2 2H2O 147,02 g/mol |
| 0.25M MgCl2 | 5 g | MgCl2 6H2O 203,30 g/mol |
| autoclave |
TSB
95 ml
| LB | about 80 ml |
| 10% PEG (MW 3.350) | 10 g |
| 20 mM Mg++ | 10 ml of 200mM |
| autoclave | |
| 5% DMSO | 5 ml |
back to Protocols
