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Team:ESBS-Strasbourg/Transformation Barbara
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(New page: DNA in small volume (maximal 10µl) <br> + 20µl of KCM 5X <br> qsp 100µl H2O <br><br> Add 100µl of competent bacteria <br><br> Leave 20 min on ice, then 5 min at room temperature <br>...)
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(New page: DNA in small volume (maximal 10µl) <br> + 20µl of KCM 5X <br> qsp 100µl H2O <br><br> Add 100µl of competent bacteria <br><br> Leave 20 min on ice, then 5 min at room temperature <br>...)
Newer edit →
Revision as of 12:23, 14 July 2008
DNA in small volume (maximal 10µl)
+ 20µl of KCM 5X
qsp 100µl H2O
Add 100µl of competent bacteria
Leave 20 min on ice, then 5 min at room temperature
It is possible to let them grow again for 1 hour at 37°C, or plate them immediately
No thermic shock
