Team:KULeuven/14 July 2008

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We will meet at 11 o'clock in room 00.210
We will meet at 11 o'clock in room 00.210
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== Testing the system ==
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voor gebruik INPUT, gebruik/test deel 1 van Cell Death Part (luxR, TetR onder constit promotor)
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# Test input output as such (= test OmpR promotor etc)
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# MEMORY TEST
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## tot aan 2de terminator set, change c2 P22 to GFP
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## anti-LuxI --> quantitate with Q-RT-PCR (vergelijk met LuxI expressie INVERTER)
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# T7 karakterisatie (met LVA tag)
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## oftewel met Ab's (Western blot of ...), p(T7)-GFP
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# FILTER TEST = filter + p(T7)-lock3d-GFP
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# INVERTER TEST: Q-RT-PCR LuxI
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# PULSE GENERATOR TEST: aiiA --> GFP
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# CELL DEATH: Entire system with YFP ofzo ipv ccdB
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input <br>
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test met output <br>
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hang filter achter input <br>
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:# karakterisatie filter via korte pulsen
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:# test pulse generator
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maak inverter <br>
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maak memory <br>
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hang deel 2 cell death (ccdB) achter deel 1 <br>

Revision as of 12:58, 14 July 2008

< return to notebook

We will meet at 11 o'clock in room 00.210

Testing the system

voor gebruik INPUT, gebruik/test deel 1 van Cell Death Part (luxR, TetR onder constit promotor)

  1. Test input output as such (= test OmpR promotor etc)
  2. MEMORY TEST
    1. tot aan 2de terminator set, change c2 P22 to GFP
    2. anti-LuxI --> quantitate with Q-RT-PCR (vergelijk met LuxI expressie INVERTER)
  3. T7 karakterisatie (met LVA tag)
    1. oftewel met Ab's (Western blot of ...), p(T7)-GFP
  4. FILTER TEST = filter + p(T7)-lock3d-GFP
  5. INVERTER TEST: Q-RT-PCR LuxI
  6. PULSE GENERATOR TEST: aiiA --> GFP
  7. CELL DEATH: Entire system with YFP ofzo ipv ccdB


input
test met output
hang filter achter input

  1. karakterisatie filter via korte pulsen
  2. test pulse generator

maak inverter
maak memory
hang deel 2 cell death (ccdB) achter deel 1

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