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Team:KULeuven/15 July 2008
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(New page: < return to notebook == Lab Work == === Wetlab === === Dry lab === Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of ...)
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(New page: < return to notebook == Lab Work == === Wetlab === === Dry lab === Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of ...)
Newer edit →
Revision as of 14:56, 15 July 2008
Contents |
Lab Work
Wetlab
Dry lab
Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of the active site of the enzyme. We'll have to do N-terminal tagging, this could work, see the crystal structure of T7. See the link under literature on this wiki. Lon-mediated degradation of lambda excisionase (Xis) and E.coli UmuD gene product. 30-40 amino temrinal AA of UmuD should do the trick.
Modeling
Parameters, parameters, parameters, ...
Remarks
Project was checked by the presentation audience
