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Team:ESBS-Strasbourg/15 July 2008
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== WetLab == | == WetLab == | ||
| + | |||
| + | Paul: | ||
| + | -PCR of Gal4 and VP16 from the plasmid given by marielle <br> | ||
| + | conditions: 100ng plasmid, 2.5µL primers, pFusion+MgSO4 buffer 5µL, 5µL dNTP (2mM), pFusion 1u <br> | ||
| + | =>No amplifications, no trace of primers after gel migration (1g agar in 100mL, 100V) <br> | ||
| + | |||
| + | -second migration: | ||
| + | same PCR gal4 product (20 µL in gel this time) <br> | ||
| + | same PCR vp16 product (20 µL in gel this time) <br> | ||
| + | Vp16 for: 1.25µL in 25µL <br> | ||
| + | Vp16 rev: 1.25µL in 25µL <br> | ||
| + | Gal4 for: 1.25µL in 25µL <br> | ||
| + | Gal4 for: 2.5µL in 25µL <br> | ||
| + | Gal4 for: 3.75µL in 25µL <br> | ||
== General == | == General == | ||
Revision as of 14:58, 15 July 2008
Contents |
DryLab
Wiki
General Layout and content (MM)
Modeling
WetLab
Paul:
-PCR of Gal4 and VP16 from the plasmid given by marielle
conditions: 100ng plasmid, 2.5µL primers, pFusion+MgSO4 buffer 5µL, 5µL dNTP (2mM), pFusion 1u
=>No amplifications, no trace of primers after gel migration (1g agar in 100mL, 100V)
-second migration:
same PCR gal4 product (20 µL in gel this time)
same PCR vp16 product (20 µL in gel this time)
Vp16 for: 1.25µL in 25µL
Vp16 rev: 1.25µL in 25µL
Gal4 for: 1.25µL in 25µL
Gal4 for: 2.5µL in 25µL
Gal4 for: 3.75µL in 25µL
General
Quote of the day
"I think that our WetLab-Team goes swimming..." (MW)
