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EPF-Lausanne/16 July 2008
From 2008.igem.org
(New page: Today, Bart is back, we can ask more questions. Also, Alex is back as well. The cells with the Prey plasmid which have Kan resistance also have a ccdB cassette which is a lethal protein. ...)
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Revision as of 08:30, 16 July 2008
Today, Bart is back, we can ask more questions. Also, Alex is back as well.
The cells with the Prey plasmid which have Kan resistance also have a ccdB cassette which is a lethal protein. It is normal that we only recovered Prey cells in CM.
Order of the day :
1. Irina : Stock the Prey which worked, stock the pLG cells (100 uL) in glycerol. Miniprep the rest which is not stocked (at least 1mL) to get the plasmid and test the concentration. Grow the already stocked plasmid-transformed cells to do miniprep tomorrow. Try new transformation with some iGEM part which we don't need and adjust protocol
2. Gaelle and Alex : Make competent cells with the Top10 grown before (2 solutions). Check plates done yesterday and culture whichever worked, but only if the control worked.
