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Team:Paris/July 14
From 2008.igem.org
(Difference between revisions)
| Line 22: | Line 22: | ||
* 500ml of SOB ( 2 x 250ml) | * 500ml of SOB ( 2 x 250ml) | ||
* 500ml of SOC (2 x 250ml) | * 500ml of SOC (2 x 250ml) | ||
| - | * 100ml of | + | * 100ml of MgCl<sub>2</sub> (1M) |
| - | * 150ml of | + | * 150ml of CaCl<sub>2</sub> (1M) |
* 200ml of KOAC (1M) | * 200ml of KOAC (1M) | ||
Revision as of 08:50, 16 July 2008
delete
Results of the transformation :
- Positive control : puc19 (1µl; 10pg/ml) --> No transformation
- Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
- Biobricks 2008 : --> No transformation
Conclusion : Cells are competents, because there is positive transformation
Hypothesis : problem with the protocol of DNA extraction from the registery 2008
- Culture of DH5ɑ in 5ml of LB without Antibiotics
Preparation of medium and solutions :
- 1L of CCMB80
- 500ml of SOB ( 2 x 250ml)
- 500ml of SOC (2 x 250ml)
- 100ml of MgCl2 (1M)
- 150ml of CaCl2 (1M)
- 200ml of KOAC (1M)
