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Minnesota/15 July 2008
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| - | |1. '''Transform Dual Promoters:''' transform the dual promoters received from GeneArt into DH5alpha competent cells. The Dual Promoters are (1) LacI and LAMBDAcI, (2) TetR and P22mnt. Follow transformation instructions in beginning of wiki. Place dual promoter DH5alpha cells in 1mL of 2xTy broth and incubate @ 37C with mixing @ 220rpm's for 2 hours to allow cell growth. | + | |1. '''Transform Dual Promoters:''' transform the dual promoters received from GeneArt into DH5alpha competent cells. The Dual Promoters are (1) LacI and LAMBDAcI, (2) TetR and P22mnt. Follow transformation instructions in beginning of wiki. Place dual promoter DH5alpha cells in 1mL of 2xTy broth and incubate @ 37C with mixing @ 220rpm's for 2 hours to allow cell growth. Plate dual promoters on Ampicillin resistant agar plates, allow to incubate overnight @37C to promote growth. |
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|2. '''Make more 2xTy Media''' | |2. '''Make more 2xTy Media''' | ||
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|3. '''Make more DH5alpha pro competent cells''' | |3. '''Make more DH5alpha pro competent cells''' | ||
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| + | |4. '''Pick Transformed Ligated Products:''' from 07-14-2008 and place in 2mL LB cultures. Incubate for 16 hours @37C with mixing @ 220rpm's. | ||
Revision as of 15:00, 16 July 2008
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| 1. Transform Dual Promoters: transform the dual promoters received from GeneArt into DH5alpha competent cells. The Dual Promoters are (1) LacI and LAMBDAcI, (2) TetR and P22mnt. Follow transformation instructions in beginning of wiki. Place dual promoter DH5alpha cells in 1mL of 2xTy broth and incubate @ 37C with mixing @ 220rpm's for 2 hours to allow cell growth. Plate dual promoters on Ampicillin resistant agar plates, allow to incubate overnight @37C to promote growth. |
| 2. Make more 2xTy Media |
| 3. Make more DH5alpha pro competent cells |
| 4. Pick Transformed Ligated Products: from 07-14-2008 and place in 2mL LB cultures. Incubate for 16 hours @37C with mixing @ 220rpm's. |
