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Team:University of Ottawa/11 July 2008
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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PTP2 Digestion''' ::<li> Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band size...) |
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:'''PTP2 Digestion''' | :'''PTP2 Digestion''' | ||
::<li> Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes. | ::<li> Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes. | ||
| + | :'''PCR confirmation''' | ||
| + | ::<li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success. | ||
Revision as of 19:02, 16 July 2008
Contents |
Today in the Lab
Matt
- PTP2 Digestion
- Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
- PCR confirmation
- PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
