Team:University of Ottawa/14 July 2008

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Revision as of 19:13, 16 July 2008

Contents

Today in the Lab

Matt

PCR Amplification
  • Corey decided it would be best to start from the beginning and amplify the PTP2 out of Genomic DNA again. I redid the PCR and I am running it overnight.
    • Inoculation
  • 97 plasmid was inoculated from master plate so that it can glycerol stocked for tomorrow.
    • PCR Confirmation
  • Corey wanted me to do another PCR confirmation of the 97 plasmid with primers F58,F59. Expecting a band size of 2200 for this PCR.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/14_July_2008"